Flow cytometric analysis of NFKB1 expression in C2C12 cells using NFKB1 antibody (R021543, 1:2,000). Green, isotype control; red, NFKB1.

Immunocytochemical staining of C2C12 cells with NFKB1 antibody (R021543, 1:1,000). Nuclei were stained blue with DAPI; NFKB1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Immunohistochemistry was performed on paraffin-embedded mouse liver using NFKB1 antibody (R021543, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.

Immunohistochemistry was performed on paraffin-embedded mouse spleen using NFKB1 antibody (R021543, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.

Immunohistochemistry was performed on paraffin-embedded mouse kidney using NFKB1 antibody (R021543, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.

Immunohistochemistry was performed on paraffin-embedded human lymphoma using NFKB1 antibody (R021543, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.

Western blotting analysis using NFKB1 antibody (R021543). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NFKB1 antibody (R021543, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using NFKB1 antibody (R021543). NFKB1 expression in wild type (WT) and NFKB1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with NFKB1 antibody (R021543, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.