Flow cytometric analysis of Chromobox 5 expression in HepG2 cells using Chromobox 5 antibody (R021509, 1:2,000). Green, isotype control; red, Chromobox 5.

Immunocytochemical staining of HepG2 cells with Chromobox 5 antibody (R021509, 1:1,000). Nuclei were stained blue with DAPI; Chromobox 5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.

Western blotting analysis using chromobox 5 antibody (R021509). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with chromobox 5 antibody (R021509, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using chromobox 5 antibody (R021509). Chromobox 5 expression in wild-type (WT) and chromobox 5 (CBX5) knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with chromobox 5 antibody (R021509, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.