Flow cytometric analysis of PPP2R5E expression in HepG2 cells using PPP2R5E antibody (R021013, 1:2,000). Green, isotype control; red, PPP2R5E.

Immunocytochemical staining of HepG2 cells with PPP2R5E antibody (R021013, 1:1,000). Nuclei were stained blue with DAPI; PPP2R5E was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using PPP2R5E antibody (R021013). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PPP2R5E antibody (R021013, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit

Western blotting analysis using PPP2R5E antibody (R021013). PPP2R5E expression in wild type (WT) and PPP2R5E shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with PPP2R5E antibody (R021013, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Validation of PPP2R5E knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with PPP2R5E antibody (R021013, 1:2,000) and analyzed using BD flow cytometer.