Flow cytometric analysis of STAT5B expression in HepG2 cells using STAT5B antibody (R020950, 1:2,000). Green, isotype control; red, STAT5B.

Western blotting analysis using STAT5B antibody (R020950). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with STAT5B antibody (R020950, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using STAT5B antibody (R020950). STAT5B expression in wild type (WT) and STAT5B shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with STAT5B antibody (R020950, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Validation of STAT5B knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with STAT5B antibody (R020950, 1:2,000) and analyzed using BD flow cytometer.

Immunocytochemical staining of Hela cells using STAT5B antibody (R020950, 1:1,000), Top panel: wild-type (WT); Bottom panal: STAT5B shRNA knockdown (KD). Nuclei were stained blue with DAPI; STAT5B was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.