Flow cytometric analysis of TFAP2A expression in HepG2 cells using TFAP2A antibody (R020943, 1:2,000). Green, isotype control; red, TFAP2A.

Immunocytochemical staining of HepG2 cells with TFAP2A antibody (R020943, 1:1,000). Nuclei were stained blue with DAPI; TFAP2A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using TFAP2A antibody (R020943). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TFAP2A antibody (R020943, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using TFAP2A antibody (R020943). TFAP2A expression in wild type (WT) and TFAP2A shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with TFAP2A antibody (R020943, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.