Flow cytometric analysis of Bcl-2 expression in HepG2 cells using Bcl-2 antibody (R020321, 1:2,000). Green, isotype control;red, Bcl-2.

Validation of Bcl-2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Bcl-2 antibody (R020321, 1:2,000) and analyzed using BD flow cytometer.

Immunohistochemistry was performed on paraffin-embedded human tonsillitis using bcl-2 antibody (R020321, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.

Western blotting analysis using Bcl 2 antibody (R020321). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Bcl 2 antibody (R020321, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using Bcl-2 antibody (R020321). Bcl-2 expression in wild type (WT) and Bcl-2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with Bcl-2 antibody (R020321, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using Bcl-2 antibody (R020321). Bcl-2 expression in wild type (WT) and BCL2 knockout (KO) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Bcl-2 antibody (R020321, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.