Flow cytometric analysis of Ribosomal protein SA expression in HepG2 cells using Ribosomal protein SA antibody (R020175, 1:2,000). Green, isotype control; red, Ribosomal protein SA.

Immunocytochemical staining of HepG2 cells with Ribosomal protein SA antibody (R020175, 1:1,000). Nuclei were stained blue with DAPI; Ribosomal protein SA was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using ribosomal protein SA antibody (R020175). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ribosomal protein SA antibody (R020175, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using ribosomal protein SA antibody (R020175). Ribosomal protein SA expression in wild type (WT) and ribosomal protein SA (RPSA) knockout (KO) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ribosomal protein SA antibody (R020175, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.