Immunocytochemical staining of HepG2 cells with Proliferating cell nuclear antigen protein antibody(M020890, 1:1,000) . Nuclei were stained blue with DAPI; Proliferating cell nuclear antigen was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using Proliferating cell nuclear antigen antibody (M020890). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Proliferating cell nuclear antigen antibody (M020890, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using Proliferating cell nuclear antigen antibody (M020890). Proliferating cell nuclear antigen expression in wild type (WT) and Proliferating cell nuclear antigen shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Proliferating cell nuclear antigen antibody (M020890, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Flow cytometric analysis of Proliferating cell nuclear antigen expression in HepG2 cells using Proliferating cell nuclear antigen antibody (M020890, 1:2,000). Green, isotype control; red, Proliferating cell nuclear antigen.

Immunohistochemistry was performed on paraffin-embedded human breast carcinoma using proliferating cell nuclear antigen antibody (M020890, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.