Validation of JUN knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) C2C12 cells were stained with JUN antibody (M020756, 1:2,000) and analyzed using BD flow cytometer.

Flow cytometric analysis of JUN expression in HT-1080 cells using JUN antibody (M020756, 1:2,000). Green, isotype control; red, JUN.

Immunocytochemical staining of HT-1080 cells with JUN antibody (M020756, 1:1,000) . Nuclei were stained blue with DAPI; JUN was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain：Medium. Scale bar, 20 μm.

Western blotting analysis using JUN antibody (M020756). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with JUN antibody (M020756, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using JUN antibody (M020756). JUN expression in wild type (WT) and JUN shRNA knockdown (KD) C2C12 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with JUN antibody (M020756, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.