Western blotting analysis using CREB1 antibody (M020222). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CREB1 antibody (M020222, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit. CREB1, cAMP responsive element binding protein 1.

Western blotting analysis using CREB1 antibody (M020222). CREB1 expression in wild type (WT) and CREB1 knockout (KO) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with CREB1 antibody (M020222, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Immunocytochemical staining of C2C12 cells with CREB1 antibody (M020222, 1:1,000). Nuclei were stained blue with DAPI;CREB1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.