Western Blot - Anti-CD68 Mouse mAb [80M22L04]

All lanes: M012181 at 1:1,000 dilution

Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysates

Lane 2: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysates

Lane 3: HCT116 (human colorectal carcinoma epithelial cell) whole cell lysates

Lane 4: T24 (human bladder cancer epithelial cell) whole cell lysates

Lane 5: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysates

Lysates/proteins at 10 µg per lane.

Secondary antibody: Goat Anti-Mouse IgG(H+L), HRP Conjugated (Cat. No. LF101) at 1:5,000 dilution

Predicted band size: 37 kDa

Observed band size: 70-80 kDa, 120-140 kDa

Developed using the ECL technique (Cat. No. SQ201).

Immunohistochemistry - Anti-CD68 Mouse mAb [80M22L04]

Sample: Paraformaldehyde-fixed, paraffin embedded human lung cancer tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: M012181 at 1:200 dilution

Secondary antibody: Goat Anti-Mouse IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.