Immunocytochemical staining of C2C12 cells with MAP1LC3A antibody (R021887, 1:1,000). Nuclei were stained blue with DAPI; MAP1LC3A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using MAP1LC3A antibody (R021887). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MAP1LC3A antibody (R021887, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using MAP1LC3A antibody (R021887). MAP1LC3A expression in wild type (WT) and MAP1LC3A shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with MAP1LC3A antibody (R021887, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.