Flow cytometric analysis of CDKN2C expression in HeLa cells using CDKN2C antibody(R021876, 1:2,000). Green, isotype control; red, CDKN2C.

Immunocytochemical staining of Hela cells with CDKN2C antibody (R021876, 1:1,000). Nuclei were stained blue with DAPI; CDKN2C was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using CDKN2C antibody (R021876). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CDKN2C antibody (R021876, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using CDKN2C antibody (R021876). CDKN2C expression in wild type (WT) and CDKN2C shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with CDKN2C antibody (R021876, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Validation of CDKN2C knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with CDKN2C antibody (R021876, 1:2,000) and analyzed using BD flow cytometer.