Flow cytometric analysis of Histone deacetylase 9 expression in HepG2 cells using Histone deacetylase 9 antibody (R021861, 1:2,000). Green, isotype control; red, Histone deacetylase 9.

Immunocytochemical staining of HepG2 cells with Histone deacetylase 9 antibody (R021861, 1:1,000). Nuclei were stained blue with DAPI; Histone deacetylase 9 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.

Western blotting analysis using Histone deacetylase 9 antibody (R021861). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Histone deacetylase 9 antibody (R021861, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using Histone deacetylase 9 antibody (R021861). Histone deacetylase 9 expression in wild type (WT) and histone deacetylase 9 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Histone deacetylase 9 antibody (R021861, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.