Western blotting analysis using DNA PKcs antibody (R021857). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DNA PKcs antibody (R021857, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using DNA PKcs antibody (R021857). DNA PKcs expression in wild type (WT) and DNA PKcs shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with DNA PKcs antibody (R021857, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Validation of DNA PKcs knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with DNA PKcs antibody (R021857, 1:2,000) and analyzed using BD flow cytometer.

Immunocytochemical staining of Hela cells using DNA PKcs antibody (R021857, 1:1,000), Top panel: wild-type (WT); Bottom panal: DNA PKcs shRNA knockdown (KD). Nuclei were stained blue with DAPI; DNA PKcs was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.

Flow cytometric analysis of DNA PKcs expression in HepG2 cells using DNA PKcs antibody (R021857, 1:2,000). Green, isotype control; red, DNA PKcs.

Immunocytochemical staining of HepG2 cells with DNA PKcs antibody (R021857, 1:1,000). Nuclei were stained blue with DAPI; DNA PKcs was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.