Flow cytometric analysis of RPS6KA1 expression in HeLa cells using RPS6KA1 antibody (R021849, 1:2,000). Green, isotype control; red, RPS6KA1.

Immunocytochemical staining of Hela cells with RPS6KA1 antibody (R021849, 1:1,000). Nuclei were stained blue with DAPI; RPS6KA1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using RPS6KA1 antibody (R021849). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RPS6KA1 antibody (R021849, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using RPS6KA1 antibody (R021849). RPS6KA1 expression in wild type (WT) and RPS6KA1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with RPS6KA1 antibody (R021849, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.