Flow cytometric analysis of NEK2 expression in HepG2 cells using NEK2 antibody (R021848, 1:2,000). Green, isotype control; red, NEK2.

Immunocytochemical staining of HepG2 cells with NEK2 antibody (R021848, 1:1,000). Nuclei were stained blue with DAPI; NEK2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.

Western blotting analysis using NEK2 antibody (R021848). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NEK2 antibody (R021848, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using NEK2 antibody (R021848). NEK2 expression in wild type (WT) and NEK2 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with NEK2 antibody (R021848, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.