Flow cytometric analysis of Histone acetyltransferase 1 expression in HeLa cells using Histone acetyltransferase 1 antibody (R021847, 1:2,000). Green, isotype control; red, Histone acetyltransferase 1.

Immunocytochemical staining of Hela cells with Histone acetyltransferase 1 antibody (R021847, 1:1,000). Nuclei were stained blue with DAPI; Histone acetyltransferase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using Histone acetyltransferase 1 antibody (R021847). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Histone acetyltransferase 1 antibody (R021847, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using Histone acetyltransferase 1 antibody (R021847). Histone acetyltransferase 1 expression in wild type (WT) and histone acetyltransferase 1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with Histone acetyltransferase 1 antibody (R021847, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Immunocytochemical staining of HeLa cells using Histone acetyltransferase 1 antibody (R021847, 1:1,000), Top panel: wild-type (WT); Bottom panal:Histone acetyltransferase 1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Histone acetyltransferase 1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.