Flow cytometric analysis of RAD51 expression in HAP-1 cells using RAD51 antibody (R021803, 1:2,000). Green, isotype control; red, RAD51.

Immunocytochemical staining of HAP-1 cells with RAD51 antibody (R021803, 1:1,000). Nuclei were stained blue with DAPI; RAD51 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using RAD51 antibody (R021803). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RAD51 antibody (R021803, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using RAD51 antibody (R021803). RAD51 expression in wild type (WT) and RAD51 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with RAD51 antibody (R021803, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.