Flow cytometric analysis of PPP3CA expression in HeLa cells using PPP3CA antibody (R021769, 1:2,000). Green, isotype control; red, PPP3CA.

Immunocytochemical staining of HeLa cells with PPP3CA antibody (R021769, 1:1,000). Nuclei were stained blue with DAPI; PPP3CA was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using PPP3CA antibody (R021769). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PPP3CA antibody (R021769, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit. PPP3CA, protein phosphatase 3 catalytic subunit alpha.

Western blotting analysis using PPP3CA antibody (R021769). PPP3CA expression in wild type (WT) and PPP3CA shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with PPP3CA antibody (R021769, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.