Western blotting analysis using MAP3K7 antibody (R021749). MAP3K7 expression in wild type (WT) and MAP3K7 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with MAP3K7 antibody (R021749, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Immunocytochemical staining of HeLa cells using MAP3K7 antibody (R021749, 1:1,000), Top panel: wild-type (WT); Bottom panal:MAP3K7 shRNA knockdown (KD). Nuclei were stained blue with DAPI;MAP3K7 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.

Flow cytometric analysis of MAP3K7 expression in HeLa cells using MAP3K7 antibody (R021749, 1:2,000). Green, isotype control; red, MAP3K7.

Immunocytochemical staining of Hela cells with MAP3K7 antibody (R021749, 1:1,000). Nuclei were stained blue with DAPI; MAP3K7 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Very low. Scale bar: 20 μm.

Western blotting analysis using MAP3K7 antibody (R021749). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MAP3K7 antibody (R021749, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.