Immunocytochemical staining of HeLa cells with FURIN antibody (R021747, 1:1,000). Nuclei were stained blue with DAPI; FURIN was stained magenta with Alexa Fluor® 647. lmages were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Flow cytometric analysis of FURIN expression in HeLa cells using FURIN antibody (R021747, 1:2,000). Green, isotype control; red, FURIN.

Western blotting analysis using FURIN antibody (R021747). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with FURIN antibody (R021747, 1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using FURIN antibody (R021747). FURIN expression in wild-type (WT) and FURIN knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with FURIN antibody (R021747, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.