Validation of PAK2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with PAK2 antibody (R021746, 1:2,000) and analyzed using BD flow cytometer.

Flow cytometric analysis of PAK2 expression in HT-1080 cells using PAK2 antibody (R021746, 1:2,000). Green, isotype control; red, PAK2.

Immunocytochemical staining of HT-1080 cells with PAK2 antibody (R021746, 1:1,000). Nuclei were stained blue with DAPI; PAK2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.

Western blotting analysis using PAK2 antibody (R021746). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PAK2 antibody (R021746, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using PAK2 antibody (R021746). PAK2 expression in wild type (WT) and PAK2 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with PAK2 antibody (R021746, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.