Flow cytometric analysis of CAP1 expression in HT-1080 cells using CAP1 antibody (R021716, 1:2,000). Green, isotype control; red, CAP1.

Immunocytochemical staining of HT-1080 cells with CAP1 antibody (R021716, 1:1,000). Nuclei were stained blue with DAPI; CAP1  was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using CAP1 antibody (R021716). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CAP1 antibody (R021716, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using CAP1 antibody (R021716). CAP1 expression in wild type (WT) and CAP1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with CAP1 antibody (R021716, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Validation of CAP1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with CAP1 antibody (R021716, 1:2,000) and analyzed using CytoFLEX.

Immunocytochemical staining of HeLa cells using CAP1 antibody (R021716, 1:1,000), Top panel: wild-type (WT); Bottom panal: CAP1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; CAP1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.