Flow cytometric analysis of ATG9A expression in HeLa cells using ATG9A antibody (R021681, 1:2,000). Green, isotype control; red, ATG9A.

Immunocytochemical staining of Hela cells with ATG9A antibody (R021681, 1:1,000). Nuclei were stained blue with DAPI; ATG9A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using ATG9A antibody (R021681). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ATG9A antibody (R021681, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using ATG9A antibody (R021681). ATG9A expression in wild type (WT) and ATG9A shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with ATG9A antibody (R021681, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.