Flow cytometric analysis of Cyclin A1/A2 expression in HAP-1 cells using Cyclin A1/A2 antibody (R021676, 1:2,000). Green, isotype control; red, Cyclin A1/A2.

Immunocytochemical staining of HAP-1 cells with Cyclin A1/A2 antibody (R021676, 1:1,000). Nuclei were stained blue with DAPI; Cyclin A1/A2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using Cyclin A1/A2 antibody (R021676). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Cyclin A1/A2 antibody (R021676, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using Cyclin A1 antibody (R021676). Cyclin A1 expression in wild type (WT) and Cyclin A1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Cyclin A1 antibody (R021676, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.