Flow cytometric analysis of CDC25C expression in HeLa cells using CDC25C antibody (R021674, 1:2,000). Green, isotype control; red, CDC25C.

Immunocytochemical staining of Hela cells with CDC25C antibody (R021674, 1:1,000). Nuclei were stained blue with DAPI; CDC25C was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using CDC25C antibody (R021674). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CDC25C antibody (R021674, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using CDC25C antibody (R021674). CDC25C expression in wild type (WT) and CDC25C shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with CDC25C antibody (R021674, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.