Flow cytometric analysis of Huntingtin interacting protein 1 expression in C2C12 cells using Huntingtin interacting protein 1 antibody (R021640, 1:2,000). Green, isotype control; red, Huntingtin interacting protein 1.

Immunocytochemical staining of C2C12 cells with Huntingtin interacting protein 1 antibody (R021640, 1:1,000). Nuclei were stained blue with DAPI; Huntingtin interacting protein 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.

Western blotting analysis using huntingtin interacting protein 1 antibody (R021640). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with huntingtin interacting protein 1 antibody (R021640, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using huntingtin interacting protein 1 antibody (R021640). Huntingtin interacting protein 1 expression in wild-type (WT) and huntingtin interacting protein 1 (HIP1) shRNA knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with huntingtin interacting protein 1 antibody (R021640, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.