Flow cytometric analysis of Hydroxymethylbilane synthase expression in HeLa cells using Hydroxymethylbilane synthase antibody (R021637, 1:2,000). Green, isotype control; red, Hydroxymethylbilane synthase.

Western blotting analysis using hydroxymethylbilane synthase antibody (R021637). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with hydroxymethylbilane synthase antibody (R021637, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using hydroxymethylbilane synthase antibody (R021637). Hydroxymethylbilane synthase expression in wild-type (WT) and hydroxymethylbilane synthase (HMBS) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with hydroxymethylbilane synthase antibody (R021637, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Validation of Hydroxymethylbilane synthase knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Hydroxymethylbilane synthase antibody (R021637, 1:2,000) and analyzed using BD flow cytometer.