Validation of Alkaline phosphatase knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Alkaline phosphatase antibody (R021633, 1:2,000) and analyzed using BD flow cytometer.

Flow cytometric analysis of Alkaline phosphatase expression in HepG2 cells using Alkaline phosphatase antibody (R021633, 1:2,000). Green, isotype control; red, Alkaline phosphatase.

Immunocytochemical staining of HepG2 cells with Alkaline phosphatase antibody (R021633, 1:1,000). Nuclei were stained blue with DAPI; Alkaline phosphatase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using Alkaline phosphatase antibody (R021633). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Alkaline phosphatase antibody (R021633, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using Alkaline phosphatase antibody (R021633). Alkaline phosphatase expression in wild type (WT) and Alkaline phosphatase shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Alkaline phosphatase antibody (R021633, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.