Flow cytometric analysis of ARHGEF2 expression in HT-1080 cells using ARHGEF2 antibody (R021629, 1:2,000). Green, isotype control; red, ARHGEF2.

Immunocytochemical staining of HT-1080 cells with ARHGEF2 antibody (R021629, 1:1,000) . Nuclei were stained blue with DAPI; ARHGEF2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using ARHGEF2 antibody (R021629). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ARHGEF2 antibody (R021629, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using ARHGEF2 antibody (R021629). ARHGEF2 expression in wild type (WT) and ARHGEF2 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with ARHGEF2 antibody (R021629, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Immunocytochemical staining of HeLa cells using ARHGEF2 antibody (R021629, 1:1,000), Top panel: wild-type (WT); Bottom panal: ARHGEF2 shRNA knockdown (KD). Nuclei were stained blue with DAPI; ARHGEF2 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.

Validation of ARHGEF2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with ARHGEF2 antibody (R021629, 1:2,000) and analyzed using BD flow cytometer.