Flow cytometric analysis of Calpain small subunit 1 expression in HepG2 cells using Calpain small subunit 1 antibody (R021610, 1:2,000). Green, isotype control; red, Calpain small subunit 1.

Immunocytochemical staining of HepG2 cells with Calpain small subunit 1 antibody (R021610, 1:1,000). Nuclei were stained blue with DAPI; Calpain small subunit 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using Calpain small subunit 1 antibody (R021610). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Calpain small subunit 1 antibody (R021610, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using Calpain small subunit 1 antibody (R021610). Calpain small subunit 1 expression in wild type (WT) and Calpain small subunit 1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with Calpain small subunit 1 antibody (R021610, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.