Flow cytometric analysis of MUC1 expression in HeLa cells using MUC1 antibody (R021608, 1:2,000). Green, isotype control; red, MUC1.

Immunocytochemical staining of HeLa cells with MUC1 antibody (R021608, 1:1,000). Nuclei were stained blue with DAPI; MUC1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using MUC1 antibody (R021608). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MUC1 antibody (R021608, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using MUC1 antibody (R021608). MUC1 expression in wild-type (WT) and MUC1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with MUC1 antibody (R021608, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.