Flow cytometric analysis of S6K1 expression in C2C12 cells using S6K1 antibody (R021590, 1:2,000). Green, isotype control; red, S6K1.

Immunocytochemical staining of C2C12 cells with S6K1 antibody (R021590, 1:1,000). Nuclei were stained blue with DAPI; S6K1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using S6K1 antibody (R021590). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with S6K1 antibody (R021590, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using S6K1 antibody (R021590). S6K1 expression in wild-type (WT) and RPS6KB1 knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with S6K1 antibody (R021590, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.