Immunocytochemical staining of HepG2 cells with MAPK activated protein kinase 2 antibody (R021582, 1:1,000). Nuclei were stained blue with DAPI; MAPK activated protein kinase 2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using MAPK activated protein kinase 2 antibody (61485). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MAPK activated protein kinase 2 antibody (61485, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using MAPK activated protein kinase 2 antibody (R021582). MAPK activated protein kinase 2 expression in wild type (WT) and MAPK activated protein kinase 2 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with MAPK activated protein kinase 2 antibody (R021582, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Immunocytochemical staining of Hela cells using MAPK activated protein kinase 2 antibody (R021582, 1:1,000), Top panel: wild-type (WT); Bottom panal: MAPK activated protein kinase 2 shRNA knockdown (KD). Nuclei were stained blue with DAPI; MAPK activated protein kinase 2 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.

Validation of MAPK activated protein kinase 2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with MAPK activated protein kinase 2 antibody (R021582, 1:2,000) and analyzed using BD flow cytometer.

Flow cytometric analysis of MAPK activated protein kinase 2 expression in HepG2 cells using MAPK activated protein kinase 2 antibody (R021582, 1:2,000). Green, isotype control; red, MAPK activated protein kinase 2.