Flow cytometric analysis of NEK7 expression in HepG2 cells using NEK7 antibody (R021574, 1:2,000). Green, isotype control; red, NEK7.

Immunocytochemical staining of HepG2 cells with NEK7 antibody (R021574, 1:1,000). Nuclei were stained blue with DAPI; NEK7 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using NEK7 antibody (R021574). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NEK7 antibody (R021574, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using NEK7 antibody (R021574). NEK7 expression in wild type (WT) and NEK7 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with NEK7 antibody (R021574, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.