Flow cytometric analysis of PPM1G expression in HepG2 cells using PPM1G antibody (R021566, 1:2,000). Green, isotype control; red, PPM1G.

Validation of PPM1G knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with PPM1G antibody (R021566, 1:2,000) and analyzed using BD flow cytometer.

Immunocytochemical staining of HepG2 cells with PPM1G antibody (R021566, 1:1,000). Nuclei were stained blue with DAPI; PPM1G was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.

Immunohistochemistry was performed on paraffin-embedded mouse kidney using PPM1G antibody (R021566, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.

Immunohistochemistry was performed on paraffin-embedded human renal carcinoma using PPM1G antibody (R021566, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.

Western blotting analysis using PPM1G antibody (R021566). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PPM1G antibody (R021566, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using PPM1G antibody (R021566). PPM1G expression in wild type (WT) and PPM1G shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with PPM1G antibody (R021566, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.