[KD Validated] Anti-CARS1 Rabbit mAb [45R82N61]

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[KD Validated] Anti-CARS1 Rabbit mAb [45R82N61]

Purified Recombinant Rabbit Monoclonal Antibody

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货号	规格	价格
R021520
	20µL	¥738.00
	50µL	¥1380.00
	100µL	¥2280.00

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[KD Validated] Anti-CARS1 Rabbit mAb [45R82N61]

Flow cytometric analysis of Cysteinyl-tRNA synthetase 1 expression in HT-1080 cells using Cysteinyl-tRNA synthetase 1 antibody (R021520, 1:2,000). Green, isotype control; red, Cysteinyl-tRNA synthetase 1.

Immunocytochemical staining of HT-1080 cells with Cysteinyl-tRNA synthetase 1 antibody (R021520, 1:1,000). Nuclei were stained blue with DAPI; Cysteinyl-tRNA synthetase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using cysteinyl-tRNA synthetase 1 antibody (R021520). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with cysteinyl-tRNA synthetase 1 antibody (R021520, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using cysteinyl-tRNA synthetase 1 antibody (R021520). Cysteinyl-tRNA synthetase 1 expression in wild-type (WT) and cysteinyl-tRNA synthetase 1 (CARS1) knockdown (KD) HSHC cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with cysteinyl-tRNA synthetase 1 antibody (R021520, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Product Name	[KD Validated] Anti-CARS1 Rabbit mAb
Description	Purified Recombinant Rabbit Monoclonal Antibody
Application Applications Legend: WB=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IHC-F=Immunohistochemistry (Frozen) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, FC, IF (Cell)/ICC
Dilution	WB 1:1,000~1:5,000; FC 1:200~1:2,000; IF 1:100~1:1,000
Reactivity	Human, Rat
Host	Rabbit
Clonality	Monoclonal
Clone No.	45R82N61
Isotype	IgG
Label	Unconjugated
Immunogen	A synthesized peptide derived from human CARS
Format	Affinity purified monoclonal antibody supplied in PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.

Synonyms	CARS1; Cysteinyl-TRNA Synthetase 1; CARS; Cysteine TRNA Ligase1,Cytoplasmic; Cysteine--TRNA Ligase, Cytoplasmic; Cysteinyl-TRNA Synthetase; EC 6.1.1.16; Cysteine Translase; MGC:11246; EC 6.1.1; MCDDBH; CYSRS; CysRS; MDBH.
Molecular weight	Calculated MW: 85 kDa, Observed MW: 75 kDa
Uniprot ID	P49589
Gene ID	833
Storage	Shipped on wet ice. Store at -20℃. Stable for 12 months from date of receipt. Aliquoting is unnecessary for -20℃ storage.
Precautions	[KD Validated] Anti-CARS1 Rabbit mAb [45R82N61] is for research use only and not for use in diagnostic or therapeutic procedures.

Background	This gene encodes a class 1 aminoacyl-tRNA synthetase, cysteinyl-tRNA synthetase. Each of the twenty aminoacyl-tRNA synthetases catalyzes the aminoacylation of a specific tRNA or tRNA isoaccepting family with the cognate amino acid. This gene is one of several located near the imprinted gene domain on chromosome 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian and breast cancers. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Aug 2010]

Background

This gene encodes a class 1 aminoacyl-tRNA synthetase, cysteinyl-tRNA synthetase. Each of the twenty aminoacyl-tRNA synthetases catalyzes the aminoacylation of a specific tRNA or tRNA isoaccepting family with the cognate amino acid. This gene is one of several located near the imprinted gene domain on chromosome 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian and breast cancers. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Aug 2010]

Cellular Location	Cytoplasm.

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R021520 [KD Validated] Anti-CARS1 Rabbit mAb [45R82N61]

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