Flow cytometric analysis of Lamin A/C expression in HT-1080 cells using Lamin A/C antibody (R021512, 1:2,000). Green, isotype control; red, Lamin A/C.

Immunocytochemical staining of HT-1080 cells with Lamin A/C antibody (R021512, 1:1,000). Nuclei were stained blue with DAPI; Lamin A/C was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.

Western blotting analysis using Lamin A/C antibody (R021512). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Lamin A/C antibody (R021512, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using Lamin A/C antibody (R021512). Lamin A/C expression in wild type (WT) and LMNA knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Lamin A/C antibody (R021512, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.