Immunocytochemical staining of HepG2 cells with Hsp70 antibody (R021510, 1:1,000). Nuclei were stained blue with DAPI; Hsp70 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Flow cytometric analysis of Hsp70 expression in HepG2 cells using Hsp70 antibody (R021510, 1:2,000). Green, isotype control; red, Hsp70.

Western blotting analysis using hsp70 antibody (R021510). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with hsp70 antibody (R021510, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using Hsp70 antibody (R021510). Hsp70 expression in wild type (WT) and HSPA1A knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Hsp70 antibody (R021510, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.