Flow cytometric analysis of IKBKB expression in HepG2 cells using IKBKB antibody (R021499, 1:2,000). Green, isotype control; red, IKBKB.

Immunocytochemical staining of HepG2 cells with IKBK antibody (R021499, 1:1,000). Nuclei were stained blue with DAPI; IKBK was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using IKBKB antibody (R021499). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with IKBKB antibody (R021499, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using IKBKB antibody (R021499). IKBKB expression in wild type (WT) and IKBKB knockdown (KD) HSHC cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with IKBKB antibody (R021499, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.