Flow cytometric analysis of Integrin linked kinase expression in C2C12 cells using Integrin linked kinase antibody (R021495, 1:2,000). Green, isotype control; red, Integrin linked kinase.

Immunocytochemical staining of C2C12 cells with Integrin linked kinase antibody (R021495, 1:1,000). Nuclei were stained blue with DAPI; Integrin linked kinase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.

Western blotting analysis using integrin linked kinase antibody (R021495). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with integrin linked kinase antibody (R021495, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using integrin linked kinase antibody (R021495). Integrin linked kinase expression in wild-type (WT) and integrin linked kinase (DAG1) knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with integrin linked kinase antibody (R021495, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.