Flow cytometric analysis of Forkhead box O3 expression in HepG2 cells using Forkhead box O3 antibody (R021483, 1:2,000). Green, isotype control; red, Forkhead box O3.

Immunocytochemical staining of HepG2 cells with Forkhead box O3 antibody (R021483, 1:1,000). Nuclei were stained blue with DAPI; Forkhead box O3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using forkhead box O3 antibody (R021483). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with forkhead box O3 antibody (R021483, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using forkhead box O3 antibody (R021483). Forkhead box O3 expression in wild-type (WT) and forkhead box O3 (FOXO3) knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with forkhead box O3 antibody (R021483, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.