Flow cytometric analysis of Clathrin heavy chain expression in C2C12 cells using Clathrin heavy chain antibody (R021473, 1:2,000). Green, isotype control; red, Clathrin heavy chain.

Western blotting analysis using Clathrin heavy chain antibody (R021473). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Clathrin heavy chain antibody (R021473, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using Clathrin heavy chain antibody (R021473). Clathrin heavy chain expression in wild type (WT) and Clathrin heavy chain shRNA knockdown (KD) HT-1080 cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Clathrin heavy chain antibody (R021473, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Immunocytochemical staining of HepG2 cells with Clathrin heavy chain antibody (R021473, 1:1000). Nuclei were stained blue with DAPI; Clathrin heavy chain was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.