[KD Validated] Anti-ARRB1 Rabbit mAb [68E83A40]

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[KD Validated] Anti-ARRB1 Rabbit mAb [68E83A40]

Purified Recombinant Rabbit Monoclonal Antibody

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货号	规格	价格
R021443
	20µL	¥738.00
	50µL	¥1380.00
	100µL	¥2280.00

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[KD Validated] Anti-ARRB1 Rabbit mAb [68E83A40]

Western blotting analysis using Phospho-beta Arrestin 1 (S412) antibody (R021443). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Phospho-beta Arrestin 1 (S412) antibody (R021443, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using Phospho-beta Arrestin 1 (S412) antibody (R021443). Phospho-beta Arrestin 1 (S412) expression in wild type (WT) and Phospho-beta Arrestin 1 (S412) shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Phospho-beta Arrestin 1 (S412) antibody (R021443, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Flow cytometric analysis of Phospho-beta Arrestin 1 (S412) expression in HepG2 cells using Phospho-beta Arrestin 1 (S412) antibody (R021443, 1:2,000). Green, isotype control; red, Phospho-beta Arrestin 1 (S412).

Immunocytochemical staining of HepG2 cells with Phospho-beta Arrestin 1 (S412) antibody (R021443, 1:1,000). Nuclei were stained blue with DAPI; Phospho-beta Arrestin 1 (S412) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Immunocytochemical staining of HeLa cells using Phospho-beta Arrestin 1 (S412) antibody (R021443, 1:1,000), Top panel: wild-type (WT); Bottom panal: Phospho-beta Arrestin 1 (S412) shRNA knockdown (KD). Nuclei were stained blue with DAPI; Phospho-beta Arrestin 1 (S412) was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.

Validation of beta Arrestin 1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Phospho-beta Arrestin 1 (S412) antibody (R021443, 1:2,000) and analyzed using BD flow cytometer.

Product Name	[KD Validated] Anti-ARRB1 Rabbit mAb
Description	Purified Recombinant Rabbit Monoclonal Antibody
Application Applications Legend: WB=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IHC-F=Immunohistochemistry (Frozen) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, FC, IF (Cell)/ICC
Dilution	WB 1:1,000~1:5,000; FC 1:200~1:2,000; IF 1:100~1:1,000
Reactivity	Human
Host	Rabbit
Clonality	Monoclonal
Clone No.	68E83A40
Isotype	IgG
Label	Unconjugated
Immunogen	A synthesized peptide derived from human Phospho-beta Arrestin 1 (S412)
Format	Affinity purified monoclonal antibody supplied in PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.

Synonyms	ARRB1; Arrestin Beta 1; ARR1; Non-Visual Arrestin-2; Beta-Arrestin-1; Arrestin 2; Arrestin, Beta 1; Arrestin Beta-1; ARB1.
Molecular weight	Calculated MW: 47 kDa, Observed MW: 50 kDa
Uniprot ID	P49407
Gene ID	408
Storage	Shipped on wet ice. Store at -20℃. Stable for 12 months from date of receipt. Aliquoting is unnecessary for -20℃ storage.
Precautions	[KD Validated] Anti-ARRB1 Rabbit mAb [68E83A40] is for research use only and not for use in diagnostic or therapeutic procedures.

Background	Members of arrestin/beta-arrestin protein family are thought to participate in agonist-mediated desensitization of G-protein-coupled receptors and cause specific dampening of cellular responses to stimuli such as hormones, neurotransmitters, or sensory signals. Arrestin beta 1 is a cytosolic protein and acts as a cofactor in the beta-adrenergic receptor kinase (BARK) mediated desensitization of beta-adrenergic receptors. Besides the central nervous system, it is expressed at high levels in peripheral blood leukocytes, and thus the BARK/beta-arrestin system is believed to play a major role in regulating receptor-mediated immune functions. Alternatively spliced transcripts encoding different isoforms of arrestin beta 1 have been described. [provided by RefSeq, Jan 2011]

Background

Members of arrestin/beta-arrestin protein family are thought to participate in agonist-mediated desensitization of G-protein-coupled receptors and cause specific dampening of cellular responses to stimuli such as hormones, neurotransmitters, or sensory signals. Arrestin beta 1 is a cytosolic protein and acts as a cofactor in the beta-adrenergic receptor kinase (BARK) mediated desensitization of beta-adrenergic receptors. Besides the central nervous system, it is expressed at high levels in peripheral blood leukocytes, and thus the BARK/beta-arrestin system is believed to play a major role in regulating receptor-mediated immune functions. Alternatively spliced transcripts encoding different isoforms of arrestin beta 1 have been described. [provided by RefSeq, Jan 2011]

Cellular Location	Cytoplasm Nucleus Cell membrane Membrane Clathrin-coated pit Cell projection Pseudopodium Cytoplasmic vesicle Translocates to the plasma membrane and colocalizes with antagonist-stimulated GPCRs. The monomeric form is predominantly located in the nucleus. The oligomeric form is located in the cytoplasm. Translocates to the nucleus upon stimulation of OPRD1 (By similarity).

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R021443 [KD Validated] Anti-ARRB1 Rabbit mAb [68E83A40]

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