Flow cytometric analysis of Eukaryotic translation initiation factor 4E expression in HepG2 cells using Eukaryotic translation initiation factor 4E antibody (R021425, 1:2,000). Green, isotype control; red, Eukaryotic translation initiation factor 4E.

Immunocytochemical staining of HepG2 cells with Eukaryotic translation initiation factor 4E antibody (R021425, 1:1,000). Nuclei were stained blue with DAPI; Eukaryotic translation initiation factor 4E was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using eukaryotic translation initiation factor 4E antibody (R021425). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with eukaryotic translation initiation factor 4E antibody (R021425, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using eukaryotic translation initiation factor 4E antibody (R021425). Eukaryotic translation initiation factor 4E expression in wild type (WT) and eukaryotic translation initiation factor 4E (EIF4E) knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with eukaryotic translation initiation factor 4E antibody (R021425, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.