Flow cytometric analysis of RAB5C expression in HepG2 cells using RAB5C antibody (R021415, 1:2,000). Green, isotype control; red, RAB5C.

Immunocytochemical staining of HepG2 cells with RAB5C antibody (R021415, 1:1,000). Nuclei were stained blue with DAPI; RAB5C was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using RAB5C antibody (R021415). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RAB5C antibody (R021415, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using RAB5C antibody (R021415). RAB5C expression in wild type (WT) and RAB5C knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with RAB5C antibody (R021415, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.