Flow cytometric analysis of VAV2 expression in HepG2 cells using VAV2 antibody (R021406, 1:2,000). Green, isotype control; red, VAV2.

Immunocytochemical staining of HepG2 cells with VAV2 antibody (R021406, 1:1,000). Nuclei were stained blue with DAPI; VAV2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using VAV2 antibody (R021406). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with VAV2 antibody (R021406, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using VAV2 antibody (R021406). VAV2 expression in wild-type (WT) and VAV2 knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with VAV2 antibody (R021406, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.