Flow cytometric analysis of RAD50 expression in HeLa cells using RAD50 antibody (R021382, 1:2,000). Green, isotype control; red, RAD50.

Immunocytochemical staining of HeLa cells with RAD50 antibody (R021382, 1:1,000). Nuclei were stained blue with DAPI; RAD50 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using RAD50 antibody (R021382). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RAD50 antibody (R021382, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using RAD50 antibody (R021382). RAD50 expression in wild type (WT) and RAD50 shRNA knockdown (KD) HT-1080 cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with RAD50 antibody (R021382, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.