Flow cytometric analysis of Tripartite motif containing 24 expression in HepG2 cells using Tripartite motif containing 24 antibody (R021356, 1:2,000). Green, isotype control; red, Tripartite motif containing 24.

Immunocytochemical staining of HepG2 cells with Tripartite motif containing 24 antibody (R021356, 1:1,000). Nuclei were stained blue with DAPI; Tripartite motif containing 24 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using Tripartite motif containing 24 antibody (R021356). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Tripartite motif containing 24 antibody (R021356, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using Tripartite motif containing 24 antibody (R021356). Tripartite motif containing 24 expression in wild type (WT) and Tripartite motif containing 24 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with Tripartite motif containing 24 antibody (R021356, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Validation of Tripartite motif containing 24 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Tripartite motif containing 24 antibody (R021356, 1:2,000) and analyzed using BD flow cytometer.